‘MOLECULAR BIOLOGY’ All of the above techniques of investigation are themselves ‘molecular biology’ in the original sense of the term; however, the term ‘molecular biology’ has taken on the new and different meaning of ‘genetic engineering’ or ‘genetic manipulation.’ These techniques for manipulating nucleic acids in vitro (that is, outside living cells or organisms) do …
Much of the information about virus structures is highly visual in nature and is difficult to represent adequately in print. It is strongly recommended that the reader view the virus structure resources on the accompanying CD. Also, Figure 2.1 illustrates the approximate shapes and sizes of different families of viruses. Why bother to form a …
THE FUNCTION AND FORMATION OF VIRUS PARTICLES Lire la suite »
ULTRASTRUCTURAL STUDIES Ultrastructural studies can be considered under three areas: physical methods, chemical methods, and electron microscopy. Physical measurements of virus particles began in the 1930s with the earliest determinations of their proportions by filtration through colloidal membranes of various pore sizes. Experiments of this sort led to the first (rather inaccurate) estimates of the …
SEROLOGICAL/IMMUNOLOGICAL METHODS As the discipline of virology was emerging, the techniques of immunology were also developing, and, as with molecular biology more recently, the two disciplines have always been very closely linked. Understanding mechanisms of immunity to virus infections has, of course, been very important. Recently, the role that the immune system itself plays in …
Principles of Molecular Virology CELL CULTURE METHODS Cell culture began early in the twentieth century with whole-organ cultures, then progressed to methods involving individual cells, either primary cell cultures (somatic cells from an experimental animal or taken from a human patient which can be maintained for a short period in culture) or immortalized cell lines, …
In 1881, Louis Pasteur began to study rabies in animals. Over several years, he developed methods of producing attenuated virus preparations by progressively drying the spinal cords of rabbits experimentally infected with rabies which, when inoculated into other animals, would protect from challenge with virulent rabies virus. In 1885, he inoculated a child, Joseph Meister, …
Principles of Molecular Virology 01 LIVING HOST SYSTEMS Lire la suite »
Learning Objectives On completing this chapter, you should be able to: ■ Understand what a virus is and explain how viruses differ from all other organisms. ■ Summarize the history of virology and explain how the current state of our knowledge of viruses was achieved. ■ Describe the techniques most frequently used to study viruses. There is more …
Bonjour Vous allez trouver ici une vidéo qui explique L’électrophorèse ( Western Blot). Le western blot : est une méthode de biologie moléculaire permettant la détection et l’identification de protéines spécifiques dans un échantillon biologique (sérum ou autre extrait ou homogénat tissulaire) à l’aide d’anticorps dirigés contre ces protéines que l’on souhaite détecter. Le western blot permet ainsi de visualiser des protéines particulières dans un mélange …
Électrophorèse – Western Blot – Comment ça marche ? SUPER VIDEO EXPLICATIVE Lire la suite »