Virology

MOLECULAR BIOLOGY

‘MOLECULAR BIOLOGY’ All of the above techniques of investigation are themselves ‘molecular biology’ in the original sense of the term; however, the term ‘molecular biology’ has taken on the new and different meaning of ‘genetic engineering’ or ‘genetic manipulation.’ These techniques for manipulating nucleic acids in vitro (that is, outside living cells or organisms) do …

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ULTRASTRUCTURAL STUDIES

ULTRASTRUCTURAL STUDIES Ultrastructural studies can be considered under three areas: physical methods, chemical methods, and electron microscopy. Physical measurements of virus particles began in the 1930s with the earliest determinations of their proportions by filtration through colloidal membranes of various pore sizes. Experiments of this sort led to the first (rather inaccurate) estimates of the …

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SEROLOGICAL/IMMUNOLOGICAL METHODS

SEROLOGICAL/IMMUNOLOGICAL METHODS As the discipline of virology was emerging, the techniques of immunology were also developing, and, as with molecular biology more recently, the two disciplines have always been very closely linked. Understanding mechanisms of immunity to virus infections has, of course, been very important. Recently, the role that the immune system itself plays in …

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CELL CULTURE METHODS

Principles of Molecular Virology CELL CULTURE METHODS Cell culture began early in the twentieth century with whole-organ cultures, then progressed to methods involving individual cells, either primary cell cultures (somatic cells from an experimental animal or taken from a human patient which can be maintained for a short period in culture) or immortalized cell lines, …

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Principles of Molecular Virology 01 LIVING HOST SYSTEMS

In 1881, Louis Pasteur began to study rabies in animals. Over several years, he developed methods of producing attenuated virus preparations by progressively drying the spinal cords of rabbits experimentally infected with rabies which, when inoculated into other animals, would protect from challenge with virulent rabies virus. In 1885, he inoculated a child, Joseph Meister, …

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Électrophorèse – Western Blot – Comment ça marche ? SUPER VIDEO EXPLICATIVE

Bonjour Vous allez trouver ici une vidéo qui explique L’électrophorèse ( Western Blot). Le western blot :  est une méthode de biologie moléculaire permettant la détection et l’identification de protéines spécifiques dans un échantillon biologique (sérum ou autre extrait ou homogénat tissulaire) à l’aide d’anticorps dirigés contre ces protéines que l’on souhaite détecter. Le western blot permet ainsi de visualiser des protéines particulières dans un mélange …

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